HotTaq polymerase, 5'000 units, 5u/µL, 1mL, incl. buffers

HotTaq Polymerase is a chemically modified version of Taq polymerase. Specific properties of HotTaq polymerase allows to carry out a so called PCR with a "hot start" (Hot Start PCR). HotTaq polymerase increases sensitivity and specificity of PCR. It can increase reaction yield, reduce nonspecific amplification and give a chance to achieve a result in conditions that did not allow to obtain a product in normal PCR.
HotTaq polymerase is a thermostable enzyme with molecular weight of approx. 94kDa. The source of this enzyme is Thermus aquaticus. The enzyme has much narrower optimal temperature range of its activity maximum. While regular Taq polymerases are able to function at lower temperatures (down to approx. 25 °C), HotTaq elongates DNA at temperatures that are normally higher than primers' annealing. This property allows to avoid nonspecific amplification that occurs when using regular Taq. HotTaq expresses almost no activity until the temperature raises close to all Taq polymerases' optimum (about 70-74 °C). Enzime's half-life at 94 °C is approximately 45 min. The enzyme catalyzes DNA synthesis in 5'->3' direction in the presence of Mg²⁺ ions and at appropriate pH. It also has a 5'->3' exonuclease activity (this activity is expressed only on double-stranded DNA). The presence of the 5'->3' exonuclease activity makes the enzyme suitable for use in RealTime PCR. HotTaq polymerase adds extra adenines at the 3'-end of PCR products. It does not interfere with cloning. If it is necessary to obtain PCR products without extra adenines, we recommend using Pfu polymerase. The maximum length of PCR products that can be obtained using HotTaq polymerase is approx. 5,000 bp. 

To activate the enzyme the reaction mixture should be heated at 94 °C for 3 min (pre-activation stage) prior to PCR.

The enzyme is "aging" over time and should be used within 6 months from the date of purchase.

Buffers included (10 mL each):

x10 Taq Buffer, 25 mM MgCl₂
700 mM Tris-HCl (pH 8.3 at 25 °C), 166 mM (NH₄)₂SO₄, 25 mM MgCl₂

x10 Taq Buffer, without MgCl₂
700 mM Tris-HCl (pH 8.3 at 25 °C), 166 mM (NH₄)₂SO₄

25 mM MgCl₂