SileksMagNA™ Swabs DNA/RNA Isolation kit
The kit is designed for 100 procedures for the isolation of DNA and RNA from epithelial swabs / scrapes (buccal, vaginal, cervical, urethral, etc.).
- Application
- Characteristics
- Composition
- Description
- Downloads
- Additional
The kit is designed for efficient and fast (~ 40 min) isolation of DNA and RNA from epithelial swabs / scrapes (buccal, vaginal, cervical, urethral, etc.).
The principle of the method used in the kit is based on the reversible binding of nucleic acids (DNA and RNA) on the surface of magnetic particles. The isolated DNA and RNA can be used both in PCR and for any other molecular biological applications (labeling, cloning, sequencing, etc.). The kit contains all the necessary reagents and buffers. The extraction protocol can be modified for scaling, in the case where more NAs are required. For scaling, it is necessary to proportionally change the amount of reagents used. The use of magnetic particles enables the researcher to select the optimal amounts of materials used and to make the isolation procedure simple, fast and reproducible.
- Number of procedures100
- Starting materialswabs or scrapes
- DNA yielddepending on the source material
- Storage+4°C
- Transportationno special conditions required
START Buffer
Lysis & Binding Buffer
Sorbent (magnetic particles)
Wash 1 Buffer
Wash 2 Buffer
Wash 3 Buffer
Final Wash Buffer
Elution Buffer
RNA Protector
Swabs mostly contain outer layers of cells. This type of material is suitable for genomic DNA isolation. RNA expression in these samples may vary depending on many factors. The final result is determined by the proportion of live cells to dead or almost dead cells. While both types contain genomic DNA, only live cells have active RNA expression.
Scrapes contain deeper layers of epithelial cells. These type of samples best fit for RNA expression analysis. In the same time the scraping procedure is more invasive and causes some local damage to epithelium.