SileksMagNA-G™ Cell Culture DNA Isolation kit
The kit is designed for 100 procedures for the isolation of mainly genomic DNA from cell culture.
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The kit is designed for 100 procedures for the isolation of mainly genomic DNA from cell culture.
The principle of the method used in the kit is based on the reversible binding of nucleic acids (NA) on the surface of magnetic particles. The isolated NA can be used both in PCR and for any other molecular biological applications (labeling, cloning, sequencing, etc.). The kit contains all the necessary reagents and buffers. The extraction protocol can be modified for scaling, in the case where more NAs are required. For scaling, it is necessary to proportionally change the amount of reagents used. The use of magnetic particles enables the researcher to select the optimal amounts of materials used and to make the isolation procedure simple, fast and reproducible.
- Number of procedures100
- Starting materialsuspension of cells
- Starting volume100 μL (collected and resuspended cells)
- DNA yieldup to 10 μg depending on the source material
- Storage+4°C
- Transportationno special conditions required
START buffer
Lysis & Binding buffer
Sorbent (magnetic particles)
Wash 1 buffer
Wash 2 buffer
Wash 3 buffer
Wash buffer
Elution buffer
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The kit is optimized for isolation from approx. 105 cells. But some optimizations may be required in each individual case. A general advice is to use as small quantity of cells as needed to provide reliable and repeatable detection of the results. The number is usually in range from 0.5×105 to 5×105 cells per sample.
Figure to the right shows phoresis of total nucleic acids isolated from ~ 3×105 HeLa cells. The top band is genomic DNA (the gel is overloaded because of the high contents of DNA). The smeared bands and two solid bands with lower molecular weight are mostly represented by total RNA. |
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