Magnetic particles
Automation potential
Our magnetic particles provide great potential for automation of isolation procedures.
Robotic platforms like KingFisher, Tecan, Xiril-based stations and similar can be used.
If you need consultations on how to use our magnetic particles with such platforms, please, contact us.
Magnetic nanoparticles
Introduction
Magnetic nanoparticles are intended for isolation of various biological macromolecules (for instance DNA, RNA, proteins). All our nanoparticles are paramagnetic. It means that the particles begin to magnetize when an external magnetic field is applied. When magnetized, the particles are attracted to the source of the field and fall out of the suspension very fast. When the external field is removed, the particles loose their magnetization and can be easily resuspended.
The particles consist of paramagnetic core and functional surface. The core consists of iron oxide and determines the particles ability to be collected in an external magnetic field. This property allows to avoid centrifugation, that is required when working with nonmagnetic sorbents. The functional surface provides sorption ability. Depending on the type and design of the surface the particles can collect some specific kind of macromolecules while having low nonspecific sorption.
Properties summary
1. Particles have zero residual magnetization (after removal of an external magnetic field particles do not conglomerate and can be easily resuspended).
2. Particles are chemically inert and can be stored for a long time in aqueous solutions.
3. Particles can be used to automate isolation of nucleic acids, proteins, cells.
Main advantages
• centrifugation is not needed
• all operations can be done in one place
• easy to completely collect/discard the supernatant
• no risk of capturing small particles of the sorbent
• easy to scale isolation procedures
Particles description
Particles with SiO2 surface. Designed for nonspecific isolation of total nucleic acids. Depending on the type of the particles (DNA grade or RNA grade) they can isolate different kinds of nucleic acids. The particles have higher sorption capacity than usual silica sorbents (also known as glass milk) and many analogical particles from other manufacturers. Only 1 µl of these particles (50 mg/mL) is able to bind about 1 µg of genomic DNA. Using this type of particles, you can have higher DNA or RNA yield even with fewer amount of sorbent used.
Particles covered with NH2. Can be used for covalent binding of proteins and antibodies. Particles have hidrophylic shell and low nonspecific proteins sorption. The particles are modified to make it possible to immobilize various proteins on their surface. Active NH2 groups provide covalent binding of proteins and antibodies. These particles can bind about 60 µg of bovine serum albumine (BSA, 65 kDa) or about 50 µg of IgG (150 kDa) per 1 mg of particles. When working with different proteins it must be taken into account that smaller proteins can be bound in bigger amounts while bigger proteins in fewer amounts.
Particles covered with COOH. Can be used for covalent binding of proteins, antibodies and nucleotide probes with amino group at the 5'-end. Particles have hidrophylic shell and low nonspecific proteins and nucleic acids sorption.